Primary Cells or Cell Lines

1. Identify the Agent and source of all primary cells or cell lines: Click on the "+Add" button to access a list of potential agents.

   Add Biological Agent Information (Slide-In)

   1. Agent: The ellipses [...] will allow you to access an extensive list of over 1,500 primary cell lines. Use the wild card [%] to help maximize search results. Most cell lines are listed by their abbreviation, for instance Non-Human Primate is listed as NHP. However, there are some instances (such as Human Embryonic Kidney 293T instead of HEK) where the cell line is spelled out. You may need to search both versions.
   2. Biocontainment level: Select the highest appropriate biocontainment level for the selected cell line.
   3. Describe the use of the agent and any downstream assays being performed: Enter a brief summary of how the selected agent will be utilized. List any downstream assays that will be performed on this cell line, even if this is already listed elsewhere.
   4. Where are you obtaining the material from? Indicate whether you are buying this cell line from a commercial vendor or obtaining from a collaborator.
   5. Storage locations: The ellipses [...] will allow you to access an extensive list of room locations at Ohio State. Use the wild card [%] in the search field to help return any results with the building name. You may need to try different search terms when looking for a building with a longer name. You should also avoid abbreviations, for instance, the BRT is listed as the Biomedical Research Tower. Select the appropriate room location and click OK.
   6. Usage locations: Use the same room location list to enter the usage location for this specific agent. If the usage location and the storage location are in the same room, re-enter the same room number.
   7. Is agent used in animals? Answer yes or no
   8. Is agent used in humans? Answer yes or no
   9. Has the agent been modified by recombinant or synthetic DNA? Answer yes or no

2. List other mammalian species cell lines: If applicable, list other mammalian species cell lines.
3. List other non-mammalian species cell lines: If applicable, list other non-mammalian species cell lines.
4. Describe any agent-specific PPE: Indicate all PPE that will be used for this specific agent, even if this PPE is listed elsewhere in the submission.
5. What are the risks associated with the agents, and what mitigations will be used? Indicate any associated risks, such as blood-borne pathogens, and the mitigations taken to reduce those risks.
6. How will the agents be transported to and from the lab? Include the lab procedure for transportation and be sure to include any secondary containment.

Bacteria, Yeasts, Fungi, or Parasites

1. Identify microorganisms by Agent, strain, and source: Click on the "+Add" button to access a list of potential agents.

   Add Biological Agent Information (Slide-In)

   1. Agent: The ellipses [...] will allow you to access a list of over 600 bacteria, yeasts, fungi, or parasites. Use the wild card [%] in the search field when searching to help return any results with the agent name. Select the appropriate agent and click OK.
   2. Biocontainment level: Select the highest appropriate biocontainment level for the selected agent.
   3. Describe use of the agent: Enter a brief summary of how the selected agent will be utilized, even if this information is already listed elsewhere.
   4. Strain or nature of attenuated strain: Indicate if this is a wildtype or attenuated strain, and if applicable, the nature of the attenuated strain.
   5. Where are you obtaining the material from? Indicate whether your lab will be using a commercial vendor or obtaining from another lab, or collaborator.
   6. Storage locations: The ellipses [...] will allow you to access an extensive list of room locations at Ohio State. Use the wild card [%] in the search field to help return any results with the building name. You may need to try different search terms when looking for a building with a longer name. You should also avoid abbreviations, for instance, the BRT is listed as the Biomedical Research Tower. Select the appropriate room location and click OK.
   7. Usage locations: Use the same room location list to enter the usage location for this specific agent. If the usage location and the storage location are in the same room, re-enter the same room number.
   8. Is agent used in animals? Answer yes or no
   9. Is agent used in humans? Answer yes or no
   10. Has the agent been modified by recombinant or synthetic DNA? Answer yes or no

2. Describe other microorganisms: If you are using another microorganism, enter it here. Otherwise leave this field blank.
3. What are the routes of exposure and risks associated with the agents, and what mitigations will be used? Indicate potential routes of exposure to personnel working with this agent (e.g.: ingestion through hand to the mouth) and how this could be mitigated (face shields and a BSC).
4. How will the agents be transported to and from the lab? Include the lab procedure for transportation and be sure to include any secondary containment.

Viruses or Prions

1. Identify viruses or prions by strain and source: Click on the "+Add" button to access a list of potential agents.

   Add Biological Agent Information (Slide-In)

   1. Agent: The ellipses [...] will allow you to access a list of over 300 viruses or prions. Use the wild card [%] in the search field when searching to help return any results with the agent name. Select the appropriate agent and click OK.
   2. Biocontainment level: Select the highest appropriate biocontainment level for the selected agent.
   3. Describe the use of the agent: Enter a brief summary of how the selected agent will be utilized, even if this information is already listed elsewhere.
   4. Strain: If applicable, add the strain information.
   5. Where are you obtaining the material from? Indicate whether your lab will be using a commercial vendor or obtaining from another lab, or collaborator.
   6. Storage locations: The ellipses [...] will allow you to access an extensive list of room locations at Ohio State. Use the wild card [%] in the search field to help return any results with the building name. You may need to try different search terms when looking for a building with a longer name. You should also avoid abbreviations, for instance, the BRT is listed as the Biomedical Research Tower. Select the appropriate room location and click OK.
   7. Usage locations: Use the same room location list to enter the usage location for this specific agent. If the usage location and the storage location are in the same room, re-enter the same room number.
   8. Is agent used in animals? Answer yes or no
   9. Is agent used in humans? Answer yes or no
   10. Has the agent been modified by recombinant or synthetic DNA? Answer yes or no
   11. If this is replication incompetent lentiviral vector, Adenovirus (AV) or Adeno-associated virus (AAV), describe the nature of deficiency of prevention of viral replication (separate genome, polymerase, envelope plasmids, self-inactivating LTR, etc.): Enter this field if applicable.
   12. If applicable, viral envelope pseudotyping (e.g. VSVg): Enter this field if applicable.

2. Describe other microorganisms: If you are using another microorganism, enter it here. Otherwise leave this field blank.
3. What are the routes of exposure and risks associated with the agents, and what mitigations will be used? Indicate potential routes of exposure to personnel working with this agent (e.g.: needle stick) and how this could be mitigated (BSC, PPE and safety needles).
4. How will the agents be transported to and from the lab? Include the lab procedure for transportation and be sure to include any secondary containment.

Select Agents or Toxins

1. Identify select agents or toxins by strain and source: Click on the "+Add" button to access a list of potential agents.

   Add Biological Agent Information (Slide-In)

   1. Agent: The ellipses [...] will allow you to access a list of over 60 agents or toxins. Use the wild card [%] in the search field when searching to help return any results with the agent name. Select the appropriate agent and click OK.
   2. Biocontainment level: Select the highest appropriate biocontainment level for the selected agent.
   3. Describe the use of the agent: Enter a brief summary of how the selected agent will be utilized, even if this information already listed elsewhere.
   4. Supplier: There is only one supplied for this type of materials, BEI Resources. Indicate whether you are obtaining this from BEI Resources, or from a collaborator.
   5. Quantity: Indicate the total quantity of this agent.
   6. Personnel that will be handling the material: Use the ellipsis to add the names of any personnel that will be handling the material in any capacity.
   7. Experimental concentration: Indicate the experimental concentration (e.g. 2 e6 CFU/mL).
   8. Is agent used in animals? Answer yes or no
   9. If this is a select agent or toxin, has it been registered with DSAT? Answer yes or no
   10. Is agent used in humans? Answer yes or no
   11. Is agent recombinant or synthetic? Answer yes or no

2. Does this protocol involve an excluded select agent or toxin? Answer yes or no
3. Does this protocol involve any toxins within permissible amounts? Answer yes or no
4. Provide CDC or USDA select agent registration number(s): You must provide a CDC or USDA select agent registration number.
5. What downstream assays will you be performing? Indicate if any downstream assays will be performed on this material.
6. What are the routes of exposure and risks associated with the agents, and what mitigations will be used? Add all potential routes of exposure associated with these agents, then include mitigations including PPE and engineering controls.
7. How will the agents be transported to and from the lab? Include the lab procedure for transportation and be sure to include any secondary containment.

Biohazards Summary

This summary page is populated from the biological agents information entered on any of the tabs in the "Agents, Toxins, & Microorganisms" section of the submission. Review the summary information on this page and confirm:

1. Agent: Confirm all agent names are listed correctly. If you realize an error, you can click on the category page, select agent, and click "update" to correct.
2. BSL: Review the summary to ensure that the highest appropriate biosafety level is listed for each selected agent.
3. Type: Confirm the type of agent listed for each item in your list.
4. Select agent: This will only appear if you have a select agent listed; for most submissions this will not be applicable.
5. Storage Locations: Confirm all storage locations are listed correctly and have the appropriate room number.
6. Usage Locations: Confirm all usage locations are listed correctly and have the appropriate room number.
7. Supplier: This will only appear if you have entered a select agent or toxin. Confirm the supplier for the select agent or toxin.
8. Qt: This quantity will only appear if you have entered a select agent or toxin. Confirm the quantity for the select agent or toxin.
9. Exc: The experimental concentration will only appear if you have entered a select agent or toxin. Confirm the experimental concentration for the select agent or toxin.
10. Recombinant: Confirm that you have correctly entered yes or no for each agent.
11. Used in Animals: Confirm that you have correctly entered yes or no for each agent.
12. Used in Humans: Confirm that you have correctly entered yes or no for each agent.